tMCR‐ALS method for the determination of water concentration profiles in the stratum corneum of untreated and treated skin in vivo

Abstract Skin moisturizing is important in dermatology and cosmetics. Raman microspectroscopy a well‐suited method to determine water concentration profiles the stratum corneum (SC) of untreated formulation‐treated skin vivo. Until now, SC was quantified using ratio proteins, other components were neglected. Here, we apply tailored multivariate curve resolution‐alternating least squares (tMCR‐ALS) improve quantitative determination cosmetic oil‐treated accounting spectra lipids, oils high wavenumber region. We compared petrolatum‐treated calculated tMCR‐ALS two existing conventional extended methods, which are both based on analysis water‐to‐protein‐ratio. The results show that skin, calculates an incorrect profile depth 0–70% thickness; shows 0–10% applied results, coincide well with modern knowledge effect SC. Almond, jojoba, paraffin exhibit similar manifests itself increase intermediate depth. However, petrolatum more pronounced. In context treated proposed throughout correctly thus has great potential